snai2 mouse monoclonal otia6  (Novus Biologicals)

Journal: Cell reports

Article Title: Breast cancer cell mesenchymal transition and metastasis directed by DAP5/eIF3d-mediated selective mRNA translation

doi: 10.1016/j.celrep.2023.112646

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human/mouse Snail2/Slug antibody , Cell Signaling Technology , Cat# 9585/RRID:AB_2239535.

Techniques: Recombinant, Staining, Protease Inhibitor, Virus, Reverse Transcription, SYBR Green Assay, Bicinchoninic Acid Protein Assay, MTT Assay, Software, Imaging

Journal: iScience

Article Title: N6-methyladenosine modification of B7-H3 mRNA promotes the development and progression of colorectal cancer

doi: 10.1016/j.isci.2024.108956

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Slug , OriGene , Cat#TA800167; RRID: AB_2625278.

Techniques: Recombinant, Software

Journal: Pathology, research and practice

Article Title: FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma.

doi: 10.1016/j.prp.2023.154836

Figure Lengend Snippet: Fig. 1. Expression and correlation of FSCN1 and selected EMT transcription factors in human PDAC. (A) Kaplan-Meier curves of survival data extracted from cBioPortal with p-value determined by log-rank test. High expression defined as 2 standard deviations above the mean. n = 177. (B) Histograms of expression and Spearman correlation scatterplots with linear regression lines (grey areas represent the 95% confidence intervals) for correlation between mRNA z-scores (RNA-seq V2 RSEM) of FSCN1, TWIST1, SLUG/SNAI2 and TCF3 in human PDAC, with Spearman correlation (rho).

Article Snippet: Sections were obtained from the Mayo Clinic Pancreatic Cancer Spore and stained with haematoxylin and eosin and by immunohistochemistry with the following antibodies: FSCN1 mouse monoclonal (Agilent, M3567, 1:150), SNAI2 mouse monoclonal OTIA6 (Novus Biologicals NBP2–03886, 1:400), TWIST1 mouse monoclonal 10E4E6 (Novus Biologicals NBP2–37364, 1:1000).

Techniques: Expressing, RNA Sequencing

Journal: Pathology, research and practice

Article Title: FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma.

doi: 10.1016/j.prp.2023.154836

Figure Lengend Snippet: Fig. 2. Immunohistochemistry of FSCN1, SLUG/SNAI2 and TWIST1 in human pancreas, PanIN and PDAC. Representative images showing expression of FSCN1 (top row), SLUG/SNAI2 (middle row) and TWIST1 (bottom row) in normal pancreas, ducts graded as PanIN1–3 and PDAC (see Supplementary Table S1 for numbers of cores assessed per grade). PanIN1 and PanIN2 are equivalent to low grade PanIN and PanIN3 equivalent to high grade PanIN in the Baltimore consensus classifi cation. Boxes highlight areas in zoom images. Zoom images (last column) show detail of location of staining for FSCN1, SLUG/SNAI2 and TWIST1 in ductal epithelium. Black scale bars = 100 µm. White scale bars = 20 µm.

Article Snippet: Sections were obtained from the Mayo Clinic Pancreatic Cancer Spore and stained with haematoxylin and eosin and by immunohistochemistry with the following antibodies: FSCN1 mouse monoclonal (Agilent, M3567, 1:150), SNAI2 mouse monoclonal OTIA6 (Novus Biologicals NBP2–03886, 1:400), TWIST1 mouse monoclonal 10E4E6 (Novus Biologicals NBP2–37364, 1:1000).

Techniques: Immunohistochemistry, Expressing, Staining

Journal: Pathology, research and practice

Article Title: FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma.

doi: 10.1016/j.prp.2023.154836

Figure Lengend Snippet: Fig. 3. TMA histoscores ANOVA and overall correlation. (A): Tukey boxplots (minimum value, first quartile, median, third quartile, maximum value) of IHC his toscores by duct type with results of ANOVA analysis (n = 11–52, see Supplementary Table S1 for numbers of cores assessed per grade). PanIN1 and PanIN2 are equivalent to low grade PanIN and PanIN3 equivalent to high grade PanIN in the Baltimore consensus classification. Nuc = nuclear, cyt = cytoplasmic. *p < 0.05, * *p < 0.01, * **p < 0.001, * ** *p < 0.0001 (Tukey’s post-hoc test). (B): Heatmap showing correlation of FSCN1, TWIST1 and SLUG/SNAI2 (cytoplasmic and nuclear) histoscores. (C): Histograms of expression and Spearman correlation scatterplots with linear regression lines (grey areas represent the 95% confidence intervals) for correlation between histoscores of FSCN1, TWIST1 and SLUG/SNAI2 with Spearman correlation (rho).

Article Snippet: Sections were obtained from the Mayo Clinic Pancreatic Cancer Spore and stained with haematoxylin and eosin and by immunohistochemistry with the following antibodies: FSCN1 mouse monoclonal (Agilent, M3567, 1:150), SNAI2 mouse monoclonal OTIA6 (Novus Biologicals NBP2–03886, 1:400), TWIST1 mouse monoclonal 10E4E6 (Novus Biologicals NBP2–37364, 1:1000).

Techniques: Expressing

Journal: Pathology, research and practice

Article Title: FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma.

doi: 10.1016/j.prp.2023.154836

Figure Lengend Snippet: Fig. 4. TMA histoscores correlation by duct type. Histograms of expression and Spearman correlation scatterplots with linear regression lines (grey areas represent the 95% confidence intervals) for correlation between histoscores of FSCN1, TWIST1 and SLUG/SNAI2 with Spearman correlation (rho). PanIN1 and PanIN2 are equivalent to low grade PanIN and PanIN3 equivalent to high grade PanIN in the Baltimore consensus classification. Nuc = nuclear, cyt = cytoplasmic.

Article Snippet: Sections were obtained from the Mayo Clinic Pancreatic Cancer Spore and stained with haematoxylin and eosin and by immunohistochemistry with the following antibodies: FSCN1 mouse monoclonal (Agilent, M3567, 1:150), SNAI2 mouse monoclonal OTIA6 (Novus Biologicals NBP2–03886, 1:400), TWIST1 mouse monoclonal 10E4E6 (Novus Biologicals NBP2–37364, 1:1000).

Techniques: Expressing